Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification
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چکیده
منابع مشابه
Rapid and sensitive detection of canine distemper virus by real-time reverse transcription recombinase polymerase amplification
BACKGROUND Canine distemper, caused by Canine distemper virus (CDV), is a highly contagious and fatal systemic disease in free-living and captive carnivores worldwide. Recombinase polymerase amplification (RPA), as an isothermal gene amplification technique, has been explored for the molecular detection of diverse pathogens. METHODS A real-time reverse transcription RPA (RT-RPA) assay for the...
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28 Yam mosaic virus (YMV; genus Potyvirus) is considered to cause the most economically important 29 viral disease of yams (Dioscorea spp.) in West Africa which is the dominant region for yam 30 production globally. 31 Yams are a vegetatively propagated crop and the use of virus-free planting material forms an essential 32 component of disease control. Current serological and PCR-based diagnost...
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BACKGROUND Chikungunya virus (CHIKV) is a mosquito-borne virus currently transmitted in about 60 countries. CHIKV causes acute flu-like symptoms and in many cases prolonged musculoskeletal and joint pain. Detection of the infection is mostly done using RT-RCR or ELISA, which are not suitable for point-of-care diagnosis. METHODOLOGY/PRINCIPAL FINDINGS In this study, a reverse transcription rec...
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A method for the rapid diagnosis of early dengue virus (DENV) infection is highly needed. Here, a prototype reverse transcription-recombinase polymerase amplification (RT-RPA) assay was developed. The assay detected DENV RNA in <20 min without the need for thermocycling amplification. The assay enabled the detection of as few as 10 copies of DENV RNA. The designed RT-RPA primers and exo probe d...
متن کاملA Portable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of Foot-and-Mouth Disease Virus
Foot-and-mouth disease (FMD) is a trans-boundary viral disease of livestock, which causes huge economic losses and constitutes a serious infectious threat for livestock farming worldwide. Early diagnosis of FMD helps to diminish its impact by adequate outbreak management. In this study, we describe the development of a real-time reverse transcription recombinase polymerase amplification (RT-RPA...
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ژورنال
عنوان ژورنال: The Plant Pathology Journal
سال: 2018
ISSN: 1598-2254,2093-9280
DOI: 10.5423/ppj.nt.06.2018.0108